Responsive fluorescent nucleotides serve as efficient substrates to probe terminal uridylyl transferase

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dc.contributor.authorGEORGE, JERRIN THOMASen_US
dc.contributor.authorSRIVATSAN, SEERGAZHI G.en_US
dc.contributor.departmentDept. of Chemistryen_US
dc.date.accessioned2020-09-28T08:23:13Z
dc.date.available2020-09-28T08:23:13Z
dc.date.issued2020-10en_US
dc.description.abstractWe repurposed a terminal uridylyl transferase enzyme to site-specifically label RNA with microenvironment sensing fluorescent nucleotide mimics, which in turn provided direct read-outs to estimate the binding affinities of the enzyme to RNA and nucleotide substrates. This enzyme–probe system provides insights into the catalytic cycle, and can facilitate the development of discovery platforms to identify robust enzyme inhibitors.en_US
dc.identifier.citationChemical Communications, 56(82), 12319-12322.en_US
dc.identifier.issn1359-7345en_US
dc.identifier.issn1364-548Xen_US
dc.identifier.sourcetitleChemical Communicationsen_US
dc.identifier.urihttp://dr.iiserpune.ac.in:8080/xmlui/handle/123456789/5076
dc.identifier.urihttps://doi.org/10.1039/D0CC05092J
dc.language.isoenen_US
dc.publication.originofpublisherForeignen_US
dc.publisherRoyal Society of Chemistryen_US
dc.subjectChemistryen_US
dc.subject2020en_US
dc.subject2020-SEP-WEEK5en_US
dc.subjectTOC-SEP-2020en_US
dc.titleResponsive fluorescent nucleotides serve as efficient substrates to probe terminal uridylyl transferaseen_US
dc.typeArticleen_US

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