Synthesis of D-Glucosamine Building Block as Required for Generating Diverse Sulfation Patterns

Abstract

Heparan sulfate is extracellular irregularly sulfonated macromolecules that influence the activities of various proteins on the cell surfaces. Hence, obtaining well-defined sequences of heparan sulfate is a huge challenging to understand structurefunctional activities. Herein, we present the design and synthesis of heparan sulfate analogs that decipher the role of sulfated patterns of heparan sulfate in biological structure and functions. To achieve this, I have synthesized glucosamine building block in such a way as to generate a library of sulfated-glucosamine patterns with amine linker. These molecules will be immobilization on microarray slides to study lectin, cytokines and chemokines binding affinities and its subsequent biological responses.