INVESTIGATING THE POTENTIAL INTERACTORS DURING PLT7-DRIVEN DNRR

Abstract

Among different regeneration models, Arabidopsis thaliana exhibits an intriguing regeneration response in which adventitious roots arise from an excised leaf explant without external hormone supplementation. This regenerative response is known as De novo root regeneration (DNRR). Recent studies have also observed that depending on whether the cut end of the excised leaf is in contact with the substrate, the leaf can either form an adventitious root or a callus to facilitate wound healing. PLETHORA(PLT) genes, also known as AINTEGUMENTA-LIKE (AIL) genes, are found to play a crucial role in plant regeneration. Overexpression of PLETHORA7/AIL7(PLT7) induces DNRR in the leaf even without the cut end being in contact with any surface. Therefore, PLT7-driven DNRR might bypass mechano-dependent cell fate. To understand how PLT7 functions in an excised leaf, it is essential to elucidate the possible interacting co-factors governing its function. So, this project aims to identify novel interacting partners of PLT7 during DNRR. These interacting partners can help us understand how PLT7-driven DNRR is induced and maintained.