Synthesis and biophysical analysis of a novel gemini surfactant with lysozyme: Industrial perspective

Abstract

Compilation of novel surfactant–protein mixtures has always been an intriguing and fascinating domain owing to their implications in industrial and cosmetic realms. In this regard, herein; we have synthesized a new cleavable gemini surfactant and investigated its influence on the structural characteristics of lysozyme via state-of-art techniques. Results have demonstrated the complex formation, unfolding and stabilization of lysozyme upon gemini surfactant combination. Docking/DFT has shown that gemini binds in the vicinity of hydrophobic residues (Trp/Tyr) and interacts via both electrostatic and hydrophobic contributions. This study may be significant to devise novel surfactant–protein mixtures for their end use at industrial stratum.