Clickable Cγ-Azido(methylene/butylene) Peptide Nucleic Acids and Their Clicked Fluorescent Derivatives: Synthesis, DNA Hybridization Properties, and Cell Penetration Studies

Abstract

Synthesis, characterization, and DNA complementation studies of clickable Cγ-substituted methylene (azm)/butylene (azb) azido PNAs show that these analogues enhance the stability of the derived PNA:DNA duplexes. The fluorescent PNA oligomers synthesized by their click reaction with propyne carboxyfluorescein are seen to accumulate around the nuclear membrane in 3T3 cells.