Characterization of the Photon Conversion Factor, Noise, and Dynamic Range of Light Microscope Detection Systems

Abstract

The protocols in this collection describe how to measure and analyze the photon conversation factor (PCF photo-electrons/count), readnoise, and dynamic range of a light microscopy detection system; which can either be a point detector or an area detector, using an in-homogeneous detector illumination scheme (as opposed to uniform illumination). The collection includes protocols on how to prepare a suitable sample for the acquisition, how to acquire data, as well as a respective analysis protocol.